Using a biological safety cabinet is easy to learn, but correct operation is a must, as it ensures that the BSC is functioning effectively, protecting both operator and biological culture.

1. Pre-operation Cleaning - Clean the area with a disinfectant solution to remove any lingering contaminants.

2. Turn on the Cabinet - Ensure the UV light is off until the chamber has a few moments to run.

3. Wear Proper PPE - Gloves, Eye Protection (goggles), lab coats, and any other relevant protective gear.

4. Perform tasks - Keep hands inside of the working area and be mindful of any moving air.

5. Turn off Equipment - Ensure the UV light is off and wait for flush time to end before turning off the unit’s fans.

Proper use and maintenance of BSC is crucial to ensure it functions effectively.